"Mr. Trump! Do not play with the lion's tail, (because) you'll regret it".
Pompeo, an arch-hawk and Iran critic, also promised U.S. support to any anti-government Iranian groups.
"WE ARE NO LONGER A COUNTRY THAT WILL STAND FOR YOUR DEMENTED WORDS OF VIOLENCE & DEATH", the President added.
The tweet was reverberating across the Mideast.
As the meme spread, one user found the ideal photo to help us imagine Mr Trump screaming the words in real life.
Iran faces increased USA pressure and looming sanctions after Trump's decision to withdraw the United States from a 2015 worldwide deal over Iran's nuclear programme.
"To our Iranian-American and Iranian friends: The Trump Administration dreams the same dreams for the people of #Iran as you do, and through our labors and God's providence they will one day come true", he tweeted.
As the New York Times noted,"last month the United States said it would impose sanctions on all exporters of Iranian oil". Needless to say, Trump's tweet has not been well-received by many Twitter users, who have taken to the social media site to slam his careless tweeting once again. "BE CAUTIOUS!", he wrote.
Trita Parsi, founder and president of the lobby group National Iranian American Council, said Pompeo's tough talk could fuel grievances, destabilise Iran and set Tehran and Washington on a collision course.
The tweet signaled an immediate escalation of tensions between Washington and Tehran, and capped a weekend of angry tweets by the President on the Russian Federation investigation and the legal problems facing his former personal attorney, Michael Cohen. "The United States is with you", Pompeo said according to prepared remarks released in advance of his speech.
Some experts say Kim is using diplomacy as a way to win outside concessions and weaken USA -led global sanctions.
That comment sounds less like a "threat" aimed at the United States, as Donald Trump seems to have interpreted it, and more of a reminder as to what a full-blown military conflict between the USA and Iran - a showdown that would unavoidably drag other serious players into the fray - would look like. That effort follows the Trump administration's decision this year to withdraw from the Iran nuclear accord, which eased economic sanctions on the Islamic Republic in return for restraints on its nuclear program.
Rouhani and Iranian Supreme Leader Ayatollah Khamenei have both rejected calls to negotiate with Trump over a replacement sanctions relief deal. "We believe in the path of happiness and progress, resistance and steadfastness in the face of enemies and those who seek our ill".
During those remarks, delivered Sunday, Pompeo compared Iran's leaders to con artists and mobsters.
In the speech at the Ronald Reagan Presidential Library and Museum, Pompeo castigated Iran's political, judicial and military leaders, accusing several by name of participating in widespread corruption. He also said the government has "heartlessly repressed its own peoples human rights, dignity and fundamental freedoms".
Pompeo said Iran's leadership is made up of "hypocritical holy men" responsible for "crooked schemes" that have hurt the country's economy and people.
The now-infamous threat came as North Korea continued to test-fire ballistic missiles, including two that demonstrated a long enough range to potentially reach the United States.